>> Strain Information
- Strain Background: EY0986
ATCC 201388: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 (S288C)
- Strain Background for RFP-tagging for Colocalization: EY0987
ATCC 201389: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 (S288C)
- Library distribution information
Strains are available individually and as collections.
TAP: Open Biosystems
- Markers for Colocalization
||ORF or stain
|ER to Golgi vesicle||Sec13|
Other localizations were determined by comparison of the GFP images to the DIC and DAPI images.
- RFP-tagged Strains for Colocalization
To obtain the strains used for the colocalization studies, please contact Peter Arvidson at firstname.lastname@example.org test
The mRFP plasmid used for construction of the strains (pFA6a-mRFP1-kanMX6) is distributed by the Dana Farber/Harvard Cancer Center DNA Resource Core Plasmid Repository. Contact Roger Tsien for authorization and a Material Transfer Agreement and then submit your request to Stephanie Mohr of the DF-HCC DNA Resource Core.
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< DF-HCC Contact Info >
< Roger Tsien Contact Info >
The sequence of the RFP plasmid used to generate the fusions is here.
The sequence of the GFP plasmid used to generate the fusions is here.